The skin is the organ which mostly manifests the changes related to the ageing process and to pathological conditions of the organism. This process becomes more evident over the years due to a gradual reduction in the thickness of the epidermis and the dermis.

The thinning of the dermis is due to a gradual reduction in the activity of the fibroblasts, and consequently to an impoverishment of all the components in the extracellular matrix (collagen, elastin and hyaluronic acid) that support the epidermis and donate resistance and elasticity to it.

Thanks to Fibroskill, it is possible to preserve one's own fibroblasts interrupting the physiological ageing process the organism undergoes: this way, the young frozen cells, are able to recuperate the same cellular activity they possessed at the moment of freezing.

Fibroskill is obtained from the extraction of fibroblasts from an extremely small section of skin of about 3x3 mm from behind the ear. The sample is then put in a special kit and is sent to the Bioscience Institute laboratories.

The Fibroskill protocol foresees expansion activities aimed at multiplying the cells until they reach the ideal concentration to favour their survival and vitality.

After about two weeks, the laboratory is able to produce a 3 ml vial containing the suspension made up of vitamins, minerals and autologous fibroblasts.

The menopause is part of a biological process in which the ovaries begin to produce low levels of sexual hormones, estrogen and progesterone. The estrogens are the main female sexual hormones and promote the development of secondary sexual characters in women, they control the ovulation cycle and are involved in many aspects of the physical and emotional health of women. The progesterone mainly controls the menstrual cycle and prepares the uterus to receive the fertilized egg.

The changes involved in the menopause processes are numerous and also involve organs as the skin making it less compact and elastic. These changes are mainly related the low levels of estrogens: those, in fact, influence the production of collagen from the fibroblasts, cells located in the dermis that contribute to the formation of the connective tissue fibers.

These matrix fibers, including collagen, confer strength and elasticity to the skin and all processes of physiological aging, that involve on one hand an important reduction of the fibroblasts in the dermis and on the other hand reduced levels of estrogens, result in decreased skin elasticity and thickness. Moreover, a lack of estrogens provokes a reduction in skin hydration as well as a reduction of vascularization and in the number of fibroblasts of the dermis.

Anti-Aging

The treatment is carried out by injecting a high concentration of fibroblasts into the superficial dermis with a microscopic needle, avoiding pain or temporary imperfections.

At the end of the session, the return to every-day life is immediate and free from any discomfort. Results, visible in the following weeks, will follow the gradual increase of the collagen produced by the physiological activity of the great number of fibroblasts implanted.

The length of the treatment is related to the life cycle of the fibroblasts and therefore lasts some years.

A part of the fibroblasts expanded in the laboratory is cryopreserved to enable subsequent treatments with cells that maintain the biological age and characteristics they had at the time the original skin sample was taken.

• Using the patient's own cells (fibroblasts) prevents any risk of rejection or contagion

• The treatment may be repeated for several years thanks to the cryopreserved cells

• The use of autologous cells guarantees absolutely natural results

• The preserved fibroblasts can also be used for the treatment of skin lesions

• Freezing interrupts the aging of the preserved fibroblasts

• From a single sample of only 3x3 mm of skin it is possible to perform about 10 Fibroskill treatments

• The effectiveness of fibroblasts clinical application is widely demonstrated in literature


SAMPLING
FIBROSKILL is obtained by 3x3 mm of skin harvested from behind the ear. The dimensions of the tissue sample are very small, this is why it is unnecessary to suture.


KIT
The sample is placed in an isothermal container which guarantees the best security, temperature and pressure conditions for the shipment of biological material.


CELL FACTORY
The kit is collected by a specialised delivery service and taken to the Bioscience Institute Cell Factory in a very short time, respecting the necessary procedures required by the.


ISOLATION - EXPANSION
The Biologists at Bioscience Institute will process the sample to isolate and expand a homogeneous population of fibroblasts respecting the maximum biological security conditions.


FREEZING - PRESERVATION
After the expansion, the cells undergo programmed freezing in order to interrupt any process of senescence and to guarantee an optimal cellular viability in the future.


USE
Cryopreserved fibroblasts will be available for defreezing and expansion at any time after 10 days notice in advance, and it will be possible to carry out about ten FIBROSKILL treatments with a high concentration of autologous fibroblasts.

Date Headline Title Author Link
2012 Mar Dermatol Surg. A Multicenter, Double-Blind, Placebo-Controlled Trial of Autologous Fibroblast Therapy (Azficel-T) for the Treatment of Nasolabial Fold Wrinkles. Smith SR, Munavalli G, Weiss R, Maslowski JM, Hennegan KP, Novak JM.
2011 Apr Laryngoscope. Injection of cultured autologous fibroblasts for human vocal fold scars, Chhetri DK et al.
2011 Mar Exp
Dermatol.
Previous chronic exogenous glucocorticoid administration in vivo does not affect functional characteristics and cellular lifespan of human skin fibroblasts in vitro. Pratsinis H, Dimozi A, Pilichos K, Tsagarakis S, Yiacoumettis AM, Kletsas D.
2011 Jan Skin Res Technol. Heat shocks enhance procollagen type I and III expression in fibroblasts in ex vivo human skin. Dams SD, de Liefde-van Beest M, Nuijs AM, Oomens CW, Baaijens FP.
2010 Oct Dermatol Surg. A phase IIa open-label dose-escalation pilot study using allogeneic human dermal fibroblasts for nasolabial folds. Lowe NJ, Lowe PL, St Clair Roberts J.
2010 Sep J Invest Dermatol  Role of Kindlin-2 in Fibroblast Functions: Implications for Wound Healing He Y, Esser P, Schacht V, Bruckner-Tuderman L, Has C.
2010 Sep Dermatology Hyaluronan Used for Intradermal Injections Is Incorporated into the Pericellular Matrix and
Promotes Proliferation in Human Skin Fibroblasts in vitro
Röck K, Fischer K, Fischer JW.
2010 Aug Mech Ageing Dev. Comparison of ex-vivo and in-vitro human fibroblast ageing models Boraldi F, Annovi G, Tiozzo R, Sommer P, Quaglino D.
2010 Jul Invest Ophthalmol
Vis Sci
Insulin growth factor promotes human corneal fibroblast network formation in vitro Berthaut AV, Mirshahi P, Benabbou N, Ducros E, Agra A, Therwath A, Legeais JM,Mirshahi M.
2010 Jun Toxicology Cigarette smoke-induced Egr-1 represses T beta R-II expression in human skin dermal fibroblasts. Ji Na Kim, Jeung Tae Jeong, Sang Hoon Jeong, Sun Ae Kim, Kyung Goo Lee, Jae Bin Shin, Young Chul Kye and Sang Wook Son
2010 Jun J Cosmet Dermatol Synergistic action of a triple peptide complex on an essential extra-cellular matrix protein exhibits significant anti-aging benefits. Byrne AJ, Al-Bader T, Kerrigan D, Hickey S, Laloeuf A, Rawlings AV
2010 May J Res Med Sci Soft tissue augmentation by autologous cultured fibroblasts transplantation for treatment of wrinkles and scars: a case series of 20 patients. Nilforoushzadeh MA et al
2010 May J Cell Physiol Reactive oxygen species control senescence-associated matrix metalloproteinase-1 through c-Jun-N-terminal kinase. Dasgupta J, Kar S, Liu R, Joseph J, Kalyanaraman B, Remington SJ, Chen C,Melendez JA
2009 Sep Biofactors Coenzyme Q10 protects against oxidative stress-induced cell death and enhances the synthesis of basement membrane components in dermal and epidermal cells Terada T, Yamanishi H, Ashida Y, Inomata S, Nishiyama T, Amano S.
2008 Cell Transplant Preliminary survival studies on autologous cultured skin fibroblasts transplantation by injection Zhao, Yuming; Wang, Jiaqi; Yan, Xiaoqing; Li, Dan; Xu, Jun
2007 Nov Arch Orthop Trauma Surg. Tissue engineering of the anterior cruciate ligament: a new method using acellularized tendon allografts and autologous fibroblasts Tischer T. et al.
2007 Nov BJU Int. Autologous myoblasts and fibroblasts for female stress incontinence: a 1-year follow-up in 123 patients Mitterberger M. et al.,
2007 Mar Dermatol Surg Autologous cultured fibroblast injection for facial contour deformities: A prospective, placebo controlled, Phase III clinical trial Performed by M.D Rovert A. Weiss et. al., Funded by Fibrocell Inc.
2007 Jan Wound Repair Regen Autologous fibroblasts to treat deep and complicated leg ulcers in diabetic patients Cavallini M. et al.,
2007 Jan J Periodontol. A randomized, double-blind, placebo-controlled study to determine the safety and efficacy of cultured and expanded autologous fibroblast injections for the treatment of interdental papillary insufficiency associated with the papilla priming procedure McGuire MK. Et al.
2006 Aesthet Surg J Restylane SubQ, a non-animal stabilized hyaluronic acid gel for soft tissue augmentation of the mid- and lower face Anders Strand,Alexiz Verpaele
2006  
Cell Tissue Bank.
Clinical results of an autologous engineered skin. Sara Llames, Eva García, Verónica García, Marcela del Río, Fernando Larcher, Jose Luis Jorcano, Eva López, Purificación Holguín, Francisca Miralles, Jesús Otero and Alvaro Meana
2005 Mar  J Biol Chem. Deletion of the PDGFR-β Gene Affects Key Fibroblast Functions Important for Wound Healing. Gao Z, Sasaoka T, Fujimori T, Oya T, Ishii Y, Sabit H, Kawaguchi M, Kurotaki Y, Naito M, Wada T, Ishizawa S, Kobayashi M, Nabeshima Y, Sasahara M
2004 Dic Otolaryngol Head Neck Surg. Lamina propria replacement therapy with cultured autologous fibroblasts for vocal fold scars Chhetri DK. et al
2000 Bull Exp Biol Med. Safety of injectable autologous human fibroblasts Keller G, Sebastian J, Lacombe U, Toft K, Lask G, Revazova E.
2000 Clin Plast Surg Autologous cultured fibroblasts as cellular therapy in plastic surgery, Boss et al
1999 Arch Facial Plast Surg. Autologous fibroblasts for treatment of facial rhytids and dermal depression: a pilot study, Watson et al.,
1999 Aug J Surg Res Intradermal injection of autologous dermal fibroblasts improves wound healing in irradiated skin Ferguson PC. et al
1998 May Dermatol Surg. Autologous human collagen and dermal fibroblasts for soft tissue augmentation West TB. Et al